Fluc circular RNA (circRNA) is a firefly luciferase-encoded circular RNA molecule engineered for stable and efficient expression in cells, organoids, or in vivo. Luciferase was originally extracted from the firefly (Photinus pyralis) and can catalyze the oxidation of luciferin to produce oxyluciferin, emitting yellow-green bioluminescence (wavelength of 550-570 nm) whose intensity directly reflects the expression level of luciferase. Unlike linear mRNA, the covalently closed structure of Fluc circRNA confers resistance to exonuclease degradation, enabling prolonged protein expression and superior performance in transient assays. Its circular topology facilitates efficient intracellular delivery and translation, with demonstrated applications in gene regulation monitoring, cell viability assays, and in vivo imaging due to its high bioluminescence output and low immunogenicity.

• Positive Control for circRNA Transfection: Validating transfection efficiency and system performance.

• Delivery System Screening: Assessing nanoparticle/circRNA formulation efficacy in delivery applications.

• Self-circularized via the PIE strategy and includes a minimal scar sequence (Exon + Spacer).

• Designed with CVB3 IRES and codon optimization to enhance translational efficiency.

• Composed entirely of unmodified nucleotides.

• Chromatography purification ensures high quality and purity (Low nicked RNA).

• Carefully controlled processes to minimize batch-to-batch variation.