CatPure™ Fluc mRNA
Catalog#: CT072
100 μg
500 μg
1 mg
bulk
Product Overview

FLuc mRNA is a firefly luciferase encoded mRNA sequence, which can express luciferase protein in cells, organoids, or in vivo. Luciferase was originally extracted from the firefly (Photinus pyralis) and can catalyze the oxidation of luciferin to produce oxyluciferin, emitting yellow-green bioluminescence (wavelength of 550-570 nm) whose intensity directly reflects the expression level of luciferase.

Applications

• Positive Control for mRNA Transfection: Validating transfection efficiency and system performance.

• Delivery System Screening: Assessing nanoparticle/mRNA formulation efficacy in delivery applications.

CATUG® CatPure™ Fluc mRNA

• Sequence optimized and selected for high expression.

• Oligo-dT purification ensures high quality and purity.

• Carefully controlled processes to minimize batch-to-batch variation.

*For Research Use Only. Not for use in diagnostic procedures.
Specifications
Description
FLuc mRNA (N1-Me-Pseudo UTP)
RNA length

1913 nt

Concentration

2 mg/mL

Storage buffer

1 mM sodium citrate, pH 6.5

Storage conditions

below - 65℃

Shipping conditions

Dry ice

Analytical Items
Test
Method
Appearance

Visual

pH

USP<791>

Content

UV

Purity (A260/A280)

UV

Purity

HPLC

Integrity

CE

Capping Efficiency

LC-MS

Poly(A) tail length and distribution

LC-MS

Residual Protein

Nano Orange

Residual dsRNA

ELISA

Residual DNA template

qPCR

Endotoxin

Gel-clot method

Testing Data
mRNA Expression in Mice
Learn more

Figure 1. CatPure™ Fluc mRNA potency in mice measured by in vivo imaging at 6hr, 12hr, and 48hr after injection.

mRNA Expression in Mice
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Figure 2. CatPure™ FLuc mRNA translation in mice via in vivo protein express assay in comparison with market competitors.

mRNA Cell-free Translation
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Figure 3. mRNA cell-free translation results by SDS-page.

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