FLuc mRNA is a firefly luciferase encoded mRNA sequence, which can express luciferase protein in cells, organoids, or in vivo. Luciferase was originally extracted from the firefly (Photinus pyralis) and can catalyze the oxidation of luciferin to produce oxyluciferin, emitting yellow-green bioluminescence (wavelength of 550-570 nm) whose intensity directly reflects the expression level of luciferase.
• Positive Control for mRNA Transfection: Validating transfection efficiency and system performance.
• Delivery System Screening: Assessing nanoparticle/mRNA formulation efficacy in delivery applications.
• Sequence optimized and selected for high expression.
• Oligo-dT purification ensures high quality and purity.
• Carefully controlled processes to minimize batch-to-batch variation.
1913 nt
2 mg/mL
1 mM sodium citrate, pH 6.5
below - 65℃
Dry ice
Visual
USP<791>
UV
UV
HPLC
CE
LC-MS
LC-MS
Nano Orange
ELISA
qPCR
Gel-clot method

Figure 1. CatPure™ Fluc mRNA potency in mice measured by in vivo imaging at 6hr, 12hr, and 48hr after injection.

Figure 2. CatPure™ FLuc mRNA translation in mice via in vivo protein express assay in comparison with market competitors.

Figure 3. mRNA cell-free translation results by SDS-page.